TXNRD1 Chemi-Luminescent ELISA Kit (Human) (OKCD04004)

Catalog No: OKCD04004

Size:96WELLS

Human TXNRD1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-103%	81-96%	93-101%	80-95%
  EDTA Plasma (n=5)	86-101%	79-99%	95-102%	95-102%
  Heparin Plasma (n=5)	84-95%	86-95%	78-104%	78-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Reductase 1 (TXNRD1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thioredoxin Reductase 1 (TXNRD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thioredoxin Reductase 1 (TXNRD1) level in the sample or standard.
• ELISA Kit Range: 41.2-30,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-94	88
  EDTA Plasma (n=5)	88-97	94
  Heparin Plasma (n=5)	96-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TXNRD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TXNRD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TXNRD1 Microplate: 96 Wells (12 x 8 Well strips)
  • TXNRD1 Lyophilized Standard: 2
  • 100X TXNRD1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.0412 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TXNRD1
• Gene Full Name: thioredoxin reductase 1
• Alias Symbols: gene associated with retinoic and IFN-induced mortality 12 protein;gene associated with retinoic and interferon-induced mortality 12 protein;GRIM-12;KM-102-derived reductase-like factor;oxidoreductase;selenoprotein TXNRD1;testis tissue sperm-binding protein Li 46a;thioredoxin reductase 1, cytoplasmic;thioredoxin reductase GRIM-12;thioredoxin reductase TR1;TR;TR1;TRXR1;TXNR.
• NCBI Gene Id: 7296
• Protein Name: Thioredoxin reductase 1, cytoplasmic
• Description of Target: Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
• Uniprot ID: Q16881
• Protein Accession #: NP_001087240
• Nucleotide Accession #: NM_001093771