TWIST1 ELISA Kit (Human) (OKCD08266)

Catalog No: OKCD08266

Size:96WELLS

TWIST1 ELISA Kit (Human) (OKCD08266) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twist Transcription Factor (TWIST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twist Transcription Factor (TWIST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twist Transcription Factor (TWIST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Twist Transcription Factor (TWIST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • TWIST1 Microplate: 96 Wells (12 x 8 Well strips)
  • TWIST1 Lyophilized Standard: 2
  • 100X TWIST1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.063ng/mL
• Publications:

  The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue. BMC Cancer. 20, 916 (2020). 32972386

• Description:
  View product citations for TWIST ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: TWIST1
• Gene Full Name: twist family bHLH transcription factor 1
• Alias Symbols: ACS3;B-HLH DNA binding protein;bHLHa38;BPES2;BPES3;class A basic helix-loop-helix protein 38;CRS;CRS1;CSO;H-twist;SCS;SWCOS;TWIST;twist basic helix-loop-helix transcription factor 1;twist homolog 1;TWIST homolog of drosophila;twist-related protein 1.
• NCBI Gene Id: 7291
• Protein Name: Twist-related protein 1
• Description of Target: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).
• Uniprot ID: Q15672
• Protein Accession #: NP_000465.1
• Nucleotide Accession #: NM_000474.3