Tubb3 Antibody (OAEE00033)

Catalog No: OAEE00033

Size:100UG

Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 ug/ml). Nuclei were stained with DAPI (blue).

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red).

Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid.
2B - Merged image of co-staining with anti-beta-tubulin (TU-06; green).
Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Porcine|Rat
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: TU-20
• Isotype: IgG1
• Host: Mouse
• Application: Flow cytometry|Immunocytochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Draberova E, Lukas Z, Ivanyi D, Viklicky V, Draber P: Expression of class III beta-tubulin in normal and neoplastic human tissues. Histochem Cell Biol. 1998 Mar;109(3):231-9.
  Jirásek T, Cipro S, Musilová A, Kubecová M, Mandys V: Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody. Indian J Med Res. 2009 Jan;129(1):89-94.
  Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M: A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation. Genes Dev. 2009 Mar 1;23(5):575-88.
  Katsetos CD, Draberova E, Smejkalova B, Reddy G, Bertrand L, de Chadarévian J-P, Legido A, Nissanov J, Baas PW, Draber P: Class III beta-Tubulin and gamma-Tubulin are Co-expressed and form Complexes in Human Glioblastoma Cells. Neurochem Res (2007) 32:1387-1398.
  Jirasek T, Pisarikova E, Viklicky V, Mandys V: Expression of class III beta-tubulin in malignant epithelial tumours: animmunohistochemical study using TU-20 and TuJ-1 antibodies. Folia Histochem Cytobiol. 2007;45(1):41-5.
  Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells.Exp Cell Res. 2004 Aug 1;298(1):218-28.
  Peknicova J, Kubatova A, Sulimenko V, Draberova E, Viklicky V, Hozak P, Draber P: Differential subcellular distribution of tubulin epitopes in boar spermatozoa:recognition of class III beta-tubulin epitope in sperm tail.Biol Reprod. 2001 Sep;65(3):672-9.
  

Target Info

• Gene Symbol: TUBB3
• Gene Full Name: tubulin beta 3 class III
• Alias Symbols: beta-4;CDCBM;CDCBM1;CFEOM3;CFEOM3A;class III beta-tubulin;FEOM3;TUBB4;tubulin beta-3 chain;tubulin beta-4 chain;tubulin beta-III;tubulin, beta 3.
• NCBI Gene Id: 10381
• Protein Name: Tubulin beta-3 chain
• Description of Target: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
• Uniprot ID: Q13509
• Protein Accession #: NP_075768.1
• Nucleotide Accession #: NM_023279.2