TUBB1 Antibody (OAAB05617)

Catalog No: OAAB05617

Size:400UL

Western blot analysis of TBB1 Antibody in CEM, MCF-7, MDA-MB231 cell lysates (35ug/lane). TBB1 (arrow) was detected using the purified Pab.

TUBB1 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

Formalin-fixed and paraffin-embedded human brain tissue reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TBB1 Antibody flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
  Description: This TBB1 antibody is generated from rabbits immunized with human TBB1 recombinant protein.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TBB1 antibody is generated from rabbits immunized with human TBB1 recombinant protein.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  TUBB1 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

• Application Info: WB: 1:2000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: TBB1 Antibody flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: TUBB1
• Alias Symbols: tubulin beta-1 chain, tubulin, beta 1.
• NCBI Gene Id: 81027
• Protein Name: Tubulin beta-1 chain|tubulin beta-1 chain [Homo sapiens]
• Description of Target: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
• Uniprot ID: Q9H4B7
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_110400.1
• Molecular Weight: 50327