TTR Antibody - middle region (OAAB03757)

Catalog No: OAAB03757

Size:400ul

Western blot analysis of TTR Antibody (Center) in HepG2 cell lysates (35ug/lane). TTR (arrow) was detected using the purified Pab.

TTR is strongly supported by BioGPS gene expression data to be expressed in HepG2

Formalin-fixed and paraffin-embedded human hepatocarcinoma with TTR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

TTR Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Cow
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC, IHC-P
  Function: Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  Cellular Location: Secreted. Cytoplasm.
  Tissue Location: Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TTR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-74 amino acids from the Central region of human TTR.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Cow; Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  TTR is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Intended Use: TTR Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: TTR Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: TTR
• Alias Symbols: CTS, TTN, ATTR, CTS1, PALB, TBPA, HEL111, HsT2651
• NCBI Gene Id: 7276
• Protein Name: Transthyretin
• Description of Target: Transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc.
• Uniprot ID: P02766
• Protein Accession #: NP_000362.1
• Molecular Weight: 15887 Da