Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKEH02432
Size:96T
Price: $625.00
SKU
OKEH02432
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TTPAL ELISA Kit (Human) (OKEH02432) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human TTPAL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TTPAL and incubated. The TTPAL found in the sample or standards competes with the biotinylated TTPAL for limited binding sites on the immobilized anti-Human TTPAL antibody. Excess unbound biotinylated TTPAL and sample or standard TTPAL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TTPAL captured in the well and inversely proportional to the amount of TTPAL which was contained in the sample or standard.
ELISA Kit Range1.56-100ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TTPAL Microplate96 Wells (12 x 8 Well strips)
TTPAL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X TTPAL Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Alpha-tocopherol transfer protein-like
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolTTPAL
Gene Full Namealpha tocopherol transfer protein like
Alias Symbolsalpha-tocopherol transfer protein-like;C20orf121;tocopherol (alpha) transfer protein-like.
NCBI Gene Id79183
Protein NameQ9BTX7
Description of TargetAlpha-tocopherol transfer protein-like
Uniprot IDhttps://www.uniprot.org/uniprot/Q9BTX7
Protein Accession #NP_001034288.1
Nucleotide Accession #NM_001039199.2
Protein Size (# AA)342
Molecular Weight39 kDa
Write Your Own Review
You're reviewing:TTPAL ELISA Kit (Human) (OKEH02432)
Your Rating