TTF1 ELISA Kit (Human) (OKCD00516)

Catalog No: OKCD00516 (Formerly GWB-KBBMN9)

Size:96WELLS

Human TTF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Termination Factor, RNA polymerase I (TTF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Termination Factor, RNA polymerase I (TTF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Termination Factor, RNA polymerase I (TTF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transcription Termination Factor, RNA polymerase I (TTF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Termination Factor, RNA polymerase I (TTF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Termination Factor, RNA polymerase I (TTF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TTF1 Microplate: 96 Wells (12 x 8 Well Strips)
  • TTF1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated TTF1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transcription Termination Factor, RNA polymerase I (TTF1). No significant cross-reactivity or interference between Transcription Termination Factor, RNA polymerase I (TTF1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TTF1
• Gene Full Name: transcription termination factor 1
• Alias Symbols: RNA polymerase I termination factor;transcription termination factor 1;Transcription termination factor I;transcription termination factor, RNA polymerase I;TTF-1;TTF-I.
• NCBI Gene Id: 7270
• Protein Name: Transcription termination factor 1
• Description of Target: Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
• Uniprot ID: Q15361
• Protein Accession #: NP_001192225
• Nucleotide Accession #: NM_001205296.1
• Protein Size (# AA): 905