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Catalog No: OCOA13699
Size:20UG
Price: $179.00
SKU
OCOA13699
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TSTA3 Protein Lysate (OCOA13699)

Datasheets/ManualsPrintable datasheet for TSTA3 Protein Lysate (OCOA13699)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionTSTA3 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolGFUS
Gene Full NameGDP-L-fucose synthase
Alias Symbols3-5 epimerase/4-reductase;FX;GDP-4-keto-6-deoxy-D-mannose epimerase-reductase;GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase;GDP-L-fucose synthase;P35B;Protein FX;red cell NADP(H)-binding protein;SDR4E1;short chain dehydrogenase/reductase family 4E, member 1;Short-chain dehydrogenase/reductase family 4E member 1;testis tissue sperm-binding protein Li 45a;tissue specific transplantation antigen 3;tissue specific transplantation antigen P35B;TSTA3.
NCBI Gene Id7264
Protein NameA0A140VKC8, NM_003313, NP_003304, Q13630
Description of TargetGDP-L-fucose synthase, GDP-L-fucose synthase isoform 2 [Homo sapiens], Homo sapiens tissue specific transplantation antigen P35B (TSTA3), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q13630
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_003304
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_003313
Molecular Weight35.7 kDa
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