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Datasheets/Manuals | Printable datasheet for OASE00140-HRP |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | Tsp232A |
Isotype | IgG1 Kappa |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB |
Additional Information | Background Info: Detects ~19kDa. Detects Tsp23, no cross-reactivity to p23. |
:: | Scientific Background: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the hsp90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated hsp90 binding of client proteins (8). Since many hsp90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). Hsp90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology. Specifically TSP23 (transcript similar p23) displays 44% and 17% amino acid identity with p23 and Sba1p respectively (10). |
:: | Certificate of Analysis: 1 ug/mL was sufficient for detection of tsp23 in 20ug of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Recombinant tsp23 |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 1:1000 (WB) |
Storage Buffer | PBS pH7.4, 50% glycerol |
Reference | 1. Johnson J.L., Beito T. G., Krco C.J., Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K., Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A., Toft D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D., Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J., Toft D.O. (2003) Cell Stress Chaperones.8: 108- 13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia 10. Freeman B.C., Felts S.J., Toft D.O., Yamamato K.R. (2000) Genes Dev. 14(4): 433-434. |
Gene Symbol | AARSD1 |
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Gene Full Name | alanyl-tRNA synthetase domain containing 1 |
Alias Symbols | AlaXp |
NCBI Gene Id | 80755 |
Uniprot ID | B9A003 |
Protein Accession # | NP_001136126.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Tsp23 Antibody : HRP (OASE00140-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Tsp23 Antibody : HRP (OASE00140-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "Tsp23 Antibody : HRP (OASE00140-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Tsp23 Antibody : HRP (OASE00140-HRP)"?
This target may also be called "AlaXp" in publications.
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What is the shipping cost for "Tsp23 Antibody : HRP (OASE00140-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Tsp23 Antibody : HRP (OASE00140-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Tsp23 Antibody : HRP (OASE00140-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Tsp23 Antibody : HRP (OASE00140-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "AARSD1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "AARSD1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "AARSD1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "AARSD1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "AARSD1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "AARSD1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.