TSP2 ELISA Kit (Human) (OKBB00295)

Catalog No: OKBB00295

Size:96 Tests

Human THBS2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human TSP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for TSP2 has been precoated onto 96-well plates. Standards (NS0,G19-I1172) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TSP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TSP2 amount of sample captured in plate.
• ELISA Kit Range: 625 pg/ml - 40,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (ng/mL)	0.56	1.29	5.84	0.62	1.36	6.47
  St. Dev.	0.04	0.08	0.32	0.05	0.1	0.42
  %CV	7.14	6.20	5.47	8.06	7.35	6.49

• ELISA Kit Component:
  • Lyophilized recombinant human E-Cadherin standard: 10 ng/tube x 2
  • Anti-human E-Cadherin Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human E-Cadherin antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 156pg/ml-10,000pg/ml
  Principle: Aviva’s human TSP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human TSP2 specific-specific polyclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TSP2 amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: Thrombospondin-2(TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum, plasma(heparin, EDTA) and human milk
• Sensitivity: <10 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine
• Reference: 1. Hirose Y, Chiba K, Karasugi T, Nakajima M, Kawaguchi Y, Mikami Y, Furuichi T, Mio F, Miyake A, Miyamoto T, Ozaki
  K, Takahashi A, Mizuta H, Kubo T, Kimura T, Tanaka T, Toyama Y, Ikegawa S (May 2008). "A functional
  polymorphism in THBS2 that affects alternative splicing and MMP binding is associated with lumbar-disc herniation".
  Am J Hum Genet 82 (5): 1122–9.
  2. LaBell, T. L.; McGookey Milewicz, D. J.; Disteche, C. M.; Byers, P. H. : Thrombospondin II: partial cDNA sequence,
  chromosome location, and expression of a second member of the thrombospondin gene family in humans.
  Genomics 12: 421-429, 1992.
  3. Bein, K; Simons M (Oct. 2000). "Thrombospondin type 1 repeats interact with matrix metalloproteinase 2. Regulation
  of metalloproteinase activity". J. Biol. Chem. (UNITED STATES) 275 (41): 32167–73.
• Specificity: Natural and recombinant human TSP2
• Immunogen: Expression system for standard: NS0; Immunogen sequence: G19-I1172
• Description: For quantitative detection of human TSP2 in cell culture supernatants, serum, plasma(heparin, EDTA) and human milk.

Target Info

• Gene Symbol: THBS2
• Gene Full Name: thrombospondin 2
• Alias Symbols: THBS2, Thrombospondin-2, TSP2_HUMAN
• NCBI Gene Id: 7058
• Protein Name: Thrombospondin-2
• Description of Target: Thrombospondin-2(TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.
• Uniprot ID: P35442
• Protein Accession #: NP_003238.2
• Nucleotide Accession #: NM_003247.3