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Catalog No: OKCD00173 (Formerly GWB-KBBYN2)
Size:96WELLS
Price: $700.00
SKU
OKCD00173
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Datasheets/ManualsPrintable datasheet for TSLP ELISA Kit (Rat) (OKCD00173)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)90-97%80-88%84-97%95-103%
EDTA plasma(n=5)97-104%99-105%81-94%87-95%
heparin plasma(n=5)96-104%78-101%90-101%80-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymic Stromal Lymphopoietin (TSLP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymic Stromal Lymphopoietin (TSLP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymic Stromal Lymphopoietin (TSLP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thymic Stromal Lymphopoietin (TSLP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)83-9286
EDTA plasma(n=5)78-8981
heparin plasma(n=5)97-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymic Stromal Lymphopoietin (TSLP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymic Stromal Lymphopoietin (TSLP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-0 Microplate96 Wells (12 x 8 Well Strips)
0 Lyophilized Standard2 x 1 ng
100X Biotinylated 0 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 5.7 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Thymic Stromal Lymphopoietin (TSLP). No significant cross-reactivity or interference between Thymic Stromal Lymphopoietin (TSLP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTSLP
Gene Full Namethymic stromal lymphopoietin
NCBI Gene Id688621
Protein NameThymic stromal lymphopoietin
Uniprot IDF1M1K7
Protein Accession #XP_008770274
Nucleotide Accession #XM_008772052.2
Protein Size (# AA)135
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