tsh Antibody (OAAI00416)

Catalog No: OAAI00416

Size:100UG

Western Blot: The full length of human TSH protein was immunoblotted by with Mouse anti TSH (Cat#OAAI00416) antibody at 1:500. An immunoreactive band is observed.

IHC: Human anterior pituitary gland tissue stained with Mouse anti TSH antibody, (Cat#OAAI00416) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Clonality: Monoclonal
• Clone: M1A10
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Additional Information: Background: The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimmers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
  Positive Control: Anterior pituitary gland
  Cellular Location: Cytoplasmic
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Immunogen: A recombinant protein from the full length of human TSH protein.
• Purification: The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
• Concentration: 100ug/200ul
• Specificity: TSHB
• Formulation: This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Reference: Wondisford,F.E., Radovick,S., Moates,J.M., Usala,S.J. and Weintraub,B.D. Isolation and characterization of the Human thyrotropin beta-subunit gene. Differences in gene structure and promoter function from murine species. J. Biol. Chem. 263 (25), 12538-12542 (1988)

Target Info

• Gene Symbol: TSHB
• Gene Full Name: thyroid stimulating hormone subunit beta
• Alias Symbols: thyroid stimulating hormone beta;thyrotropin beta chain;thyrotropin subunit beta;TSH-B;TSH-BETA.
• NCBI Gene Id: 7252
• Protein Name: Temperature-sensitive hemagglutinin tsh autotransporter
• Description of Target: The four Human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimmers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.
  
• Uniprot ID: Q47692
• Protein Accession #: WP_001080137.1; WP_011402691.1; YP_009076399.1; YP_444132.1
• Nucleotide Accession #: NC_007675.1; NG_035300.1; NZ_JSPK01000031.1
• Molecular Weight: 28