TSG101 ELISA Kit (Human) (OKCA02151)

Catalog No: OKCA02151

Size:96 Wells

Human TSG101 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	98	92-104
  Serum (n=4)	1:2	108	103-112
  Serum (n=4)	1:4	94	90-98
  Serum (n=4)	1:8	89	86-95

• ELISA Kit Principle: Aviva Systems Biology TSG101 ELISA Kit (Human) (OKCA02151) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TSG101 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TSG101 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TSG101 captured in well.
• ELISA Kit Range: 23.44 pg/mL-1,500 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	102	99-106
  EDTA plasma (n=4)	94	90-98

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-TSG101 Microplate: 96 Wells (12 x 8 Well strips)
  • TSG101 Lyophilized Standard: 2 x 1.5 ng
  • 100X Biotinylated TSG101 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 5.86 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TSG101
• Alias Symbols: TSG10, VPS23
• NCBI Gene Id: 7251
• Protein Name: Tumor susceptibility gene 101 protein
• Description of Target: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan.
• Uniprot ID: Q99816
• Protein Accession #: NP_006283
• Nucleotide Accession #: NM_006292