TSC1 Antibody (OAPB00305)

Catalog No: OAPB00305 (Formerly GWB-63ADEB)

Size:100UG

Western blot analysis of TSC1 in EL4 cell lysate with TSC1 antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.

Immunofluorescence of TSC1 in L1210 cells with TSC1 antibody at 10 ug/mL.

Immunocytochemistry of TSC1 in EL4 cells with TSC1 antibody at 2 μg/mL

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. TSC1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: TSC1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: TSC1 antibody was raised against a 15 amino acid synthetic peptide from the middle region of human TSC1. The immunogen is located within amino acids 220 - 270 of TSC1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-TSC1 (OAPB00305) antibody is Catalog # OEPB02398
• Application Info: TSC1 antibody can be used for the detection of TSC1 by Western blot at 1 ug/ml. (Optimal dilution should be determined by user)
• Reference: Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.;Inoki K, Ouyang H, Li Y, et al. Signaling by target of rapamycin proteins in cell growth control. Microbiol. Mol. Biol. Rev. 2005; 69:79-100.;Tabancay Jr AP, Gau CL, Machado IM, et al. Identification of dominant negative mutants of Rheb GTPase and their use to implicate the involvement of human Rheb in the activation of p70S6K. J. Biol. Chem. 2003; 278:39921-30.;Inoki K, Li Y, Xu T, et al. Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev. 2003; 17:1829-34.

Target Info

• Gene Symbol: TSC1
• Gene Full Name: TSC complex subunit 1
• Alias Symbols: hamartin;LAM;truncated hemartin;TSC;tuberous sclerosis 1 protein.
• NCBI Gene Id: 7248
• Protein Name: Hamartin|hamartin [Homo sapiens]
• Description of Target: In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400). Seems not to be required for TSC2 GAP activity towards RHEB (PubMed:15340059). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (PubMed:29127155). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (PubMed:29127155). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (PubMed:16464865, PubMed:29127155).
• Uniprot ID: Q92574
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAC51674
• Nucleotide Accession #: NM_000368.4; NM_001162426.1; NM_001162427.1; XM_005272211.1; XM_006717271.1
• Molecular Weight: Predicted: 128 kDa Observed: 135 kDa