Catalog No: OPCA05446
Price: $0.00
SKU
OPCA05446
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
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Datasheets/Manuals | Printable datasheet for TSAE Recombinant Protein (Escherichia coli) (OPCA05446) (OPCA05446) |
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Predicted Species Reactivity | Escherichia coli |
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Product Format | Liquid or Lyophilized powder |
Additional Information | Species Specificity Detail: Escherichia coli (strain K12) |
Reconstitution and Storage | -20°C or -80°C |
Formulation | Tris-base, 50% glycerol |
Purity | Greater than 90% as determined by SDS-PAGE. |
Peptide Sequence | MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG |
Protein Sequence | MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG |
Storage Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Source | Mammalian Cells |
Protein Range | 1-153 aa |
Tag | C-terminal hFc-tagged |
Reference | The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase. Tsui H.-C.T., Zhao G., Feng G., Leung H.-C.E., Winkler M.E. Mol. Microbiol. 11:189-202(1994) |
Gene Symbol | tsaE |
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Gene Full Name | N(6)-L-threonylcarbamoyladenine synthase, TsaE subunit |
Alias Symbols | b4168;ECK4164;N(6)-L-threonylcarbamoyladenine synthase, TsaE subunit;t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE;yjeE. |
NCBI Gene Id | 948684 |
Protein Name | tRNA threonylcarbamoyladenosine biosynthesis protein TsaE |
Description of Target | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro. |
Uniprot ID | P0AF67 |
Protein Accession # | NP_418589 |
Nucleotide Accession # | NC_000913 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 42.9 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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