TRPV1 ELISA Kit (Rat) (OKEH03613)

Catalog No: OKEH03613

Size:96T

Rat TRPV1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology TRPV1 ELISA Kit (Rat) (OKEH03613) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Trpv1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Trpv1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Trpv1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Spike Average Recovery: 80%
• ELISA Kit Reproducibility: Intra-Assay CV: ≤3.9% (n-20)
  Inter-Assay CV: ≤6.4% (n=24)
• ELISA Kit Component:
  • Trpv1 Microplate: 96 Wells (12 x 8 Well strips)
  • Trpv1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Trpv1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 41.4 pg/mL
• Specificity: Natural and recombinant Rat Transient receptor potential cation channel subfamily V member 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Trpv1
• Gene Full Name: transient receptor potential cation channel, subfamily V, member 1
• Alias Symbols: capsaicin receptor;deltaN-TRPV1;osm-9-like TRP channel 1;OTRPC1;transient receptor potential cation channel subfamily V member 1;transient receptor potential vanilloid 1 supraoptic nucleus;TRPV1_SON;vanilloid receptor 1;vanilloid receptor subtype 1;vanilloid receptor type 1 like protein 1;Vanilloid receptor type 1-like;vanilloid type 1 receptor;VR.5'sv;Vr1;Vr1l1.
• NCBI Gene Id: 83810
• Protein Name: O35433
• Description of Target: Transient receptor potential cation channel subfamily V member 1
• Uniprot ID: https://www.uniprot.org/uniprot/O35433
• Protein Accession #: NP_114188.1
• Nucleotide Accession #: NM_031982.1
• Protein Size (# AA): 838
• Molecular Weight: 95 kDa