TRPV1 ELISA Kit (Dog) (OKEH07815)

Catalog No: OKEH07815

Size:96T

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	81-90% (n = 20)	95-105% (n = 20)	94-104% (n = 20)	87-98% (n = 20)
  EDTA plasma(n=5)	98-108% (n = 20)	81-92% (n = 20)	111-121% (n = 20)	91-101% (n = 20)
  heparin plasma(n=5)	90-100% (n = 20)	102-114% (n = 20)	102-111% (n = 20)	100-110% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology TRPV1 ELISA Kit (Dog) (OKEH07815) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRPV1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TRPV1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRPV1 captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.1% (n = 20)
  Mean Inter-assay CV%: <=8.2% (n = 20)
• ELISA Kit Component:
  • TRPV1 Microplate: 96 Wells (12 x 8 Well strips)
  • TRPV1 Lyophilized Standard: 2
  • 100X Biotinylated TRPV1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL (By similarity). Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  Subcellular Location: Cell junction synapse postsynaptic cell membrane Multi-pass membrane protein Cell projection dendritic spine membrane Multi-pass membrane protein
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Dog Transient receptor potential cation channel subfamily V member 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TRPV1
• Gene Full Name: transient receptor potential cation channel subfamily V member 1
• Alias Symbols: osm-9-like TRP channel 1;OTRPC1;transient receptor potential cation channel subfamily V member 1;transient receptor potential V1;vanilloid receptor 1;VR1.
• NCBI Gene Id: 445457
• Protein Name: Q697L1
• Description of Target: Transient receptor potential cation channel subfamily V member 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q697L1
• Protein Accession #: NP_001003970.1
• Nucleotide Accession #: NM_001003970.1