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TRPM8 Antibody (OAEB00748)

100 ug
$229.00
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Gene Symbol:
TRPM8
NCBI Gene Id:
79054
Protein Name:
Transient receptor potential cation channel, subfamily M, member 8, isoform CRA_b EMBL EAW71068.1
Swissprot Id:
Q3YFM7
Protein Accession #:
NP_076985.4
Replacement Item:
This antibody may replace item sc-130903 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TRPM8.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TRPM8.
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%
Complete computational species homology data:
TRPM8 Antibody (OAEB00748)
Peptide Sequence:
C-TSEQDKDNWNGQLK
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-TRPM8 antibody - OAEB00748
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot: Preliminary experiments gave an approx 28kDa band in lysates of cell line NIH3T3 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128kDa according to NP_076985.4. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
Target Reference:
* Dhaka A, Murray AN, Mathur J, Earley TJ, Petrus MJ, Patapoutian A.; TRPM8 is required for cold sensation in mice.; Neuron. 2007 May 3;54(3):371-8.; Pubmed 17481391

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