TRPM8 Antibody (OAEB00748)

Catalog#: OAEB00748

• Size: 100 ug

• Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: ELISA
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Gene Symbol: TRPM8
• NCBI Gene Id: 79054
• Protein Name: Transient receptor potential cation channel, subfamily M, member 8, isoform CRA_b EMBL EAW71068.1
• Swissprot Id: Q3YFM7
• Protein Accession #: NP_076985.4
• Replacement Item: This antibody may replace item sc-130903 from Santa Cruz Biotechnology.
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express TRPM8.
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%
• Peptide Sequence: C-TSEQDKDNWNGQLK
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Preliminary experiments gave an approx 28kDa band in lysates of cell line NIH3T3 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128kDa according to NP_076985.4. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
• Target Reference: * Dhaka A, Murray AN, Mathur J, Earley TJ, Petrus MJ, Patapoutian A.; TRPM8 is required for cold sensation in mice.; Neuron. 2007 May 3;54(3):371-8.; Pubmed 17481391

Protocol:

Tips Information:

Computational species homology for TRPM8 antibody (OAEB00748)