- Predicted Species Reactivity:
- Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
- Product Format:
- Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
- Clonality:
- Polyclonal
- Host:
- Goat
- Application:
- ELISA
- Reconstitution and Storage:
- Aliquot and store at -20C. Minimize freezing and thawing.
- Gene Symbol:
- TRPM8
- NCBI Gene Id:
- 79054
- Protein Name:
- Transient receptor potential cation channel, subfamily M, member 8, isoform CRA_b EMBL EAW71068.1
- Swissprot Id:
- Q3YFM7
- Protein Accession #:
- NP_076985.4
- Replacement Item:
- This antibody may replace item sc-130903 from Santa Cruz Biotechnology.
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express TRPM8.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express TRPM8.
- Predicted Homology Based on Immunogen Sequence:
- Cow: 92%; Dog: 92%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%
- Complete computational species homology data:
- TRPM8 Antibody (OAEB00748)
- Peptide Sequence:
- C-TSEQDKDNWNGQLK
- Datasheets/Manuals:
- Printable datasheet for anti-TRPM8 antibody - OAEB00748
- Purity:
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Application Info:
- Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot: Preliminary experiments gave an approx 28kDa band in lysates of cell line NIH3T3 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 128kDa according to NP_076985.4. The 28kDa band was successfully blocked by incubation with the immunizing peptide. - Target Reference:
- * Dhaka A, Murray AN, Mathur J, Earley TJ, Petrus MJ, Patapoutian A.; TRPM8 is required for cold sensation in mice.; Neuron. 2007 May 3;54(3):371-8.; Pubmed 17481391
Product Reviews
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