TrpM7 Antibody (OASE00166)

Catalog No: OASE00166 (Formerly GWB-9BAC01)

Size:100ug

Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS (pH 7.4) with 50% glycerol and 0.09% sodium azide.
• Clonality: Monoclonal
• Clone: S74
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, AM
  Certificate of Analysis: 1 ug/mL was sufficient for detection of TrpM7 in 10ug of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
  Cellular Localization: Membrane
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~220kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Application Info: WB: 1:1000
  IHC: 1:1000
  ICC/IF: 1:100
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
  2. www.iochannels.org
  3. Brauchi S., Krapivinsky G., Krapivinsky L., Clapham D.E. (2008)
  Proc Natl Acad Sci USA. 105(24): 8304-8308.
  4. Numata T, Okada Y. (2008) J Biol Chem. 283(22): 15097-15103.
• Description: TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (1, 2).

Target Info

• Gene Symbol: TRPM7
• Gene Full Name: transient receptor potential cation channel, subfamily M, member 7
• Alias Symbols: CHAK, CHAK1, Channel kinase 1, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTRPC7, Transient receptor potential cation channel subfamily M member 7, TRP PLIK, TRPM7, TRPM7_HUMAN
• NCBI Gene Id: 58800
• Description of Target: TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (1, 2).
• Uniprot ID: Q923J1
• Protein Accession #: NP_001157797.1
• Molecular Weight: 220 kDa