TrpM7 Antibody : HRP (OASE00166-HRP)

Catalog No: OASE00166-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: S74-25
• Isotype: IgG1
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IP, ICC, IF
• Additional Information: Background Info: Detects ~220kDa. No cross-reactivity against TrpM6
  Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
  TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (3, 4).
  
  Certificate of Analysis: 1 ug/mL was sufficient for detection of TrpM7 in 10ug of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7, accession number Q923J1
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA.
  2. www.iochannels.org
  3. Brauchi S., Krapivinsky G., Krapivinsky L., Clapham D.E. (2008)
  Proc Natl Acad Sci USA. 105(24): 8304-8308.
  4. Numata T, Okada Y. (2008) J Biol Chem. 283(22): 15097-15103.

Target Info

• Gene Symbol: Trpm7
• Gene Full Name: transient receptor potential cation channel, subfamily M, member 7
• Alias Symbols: Lt, CHA, LTR, TRP, CHAK, CHAK1, Ltpr7, Ltrpc7, LTrpC-7, TRPPLIK, 2310022G15Rik, 4833414K03Rik, 5033407O22Rik
• NCBI Gene Id: 58800
• Uniprot ID: Q923J1
• Protein Accession #: NP_001157797.1