TRPM2 Antibody (OACA07804)

Catalog No: OACA07804

Immunohistochemistry of paraffin-embedded human pancreas tissue at dilution 1:100

Immunohistochemistry of paraffin-embedded human tonsil tissue at dilution 1:100

Western blot
All lanes: TRPM2 antibody at 2ug/ml+k562 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 172,166,96 kDa
Observed band size: 171 kDa

Western blot
All lanes: TRPM2 antibody at 2ug/ml+k562 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 172,166,96 kDa
Observed band size: 171 kDa

Immunohistochemistry of paraffin-embedded human pancreas tissue at dilution 1:100

Immunohistochemistry of paraffin-embedded human tonsil tissue at dilution 1:100

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Transient receptor potential cation channel subfamily M member 2 protein (1204-1503AA)
• Purification: Affinity Purified
• Concentration: Varies by lot. See vial for exact concentration.
• Application Info: WB: 1:200~1000
  IHC: 1:20~200
• Reference: [1]"Activation of the cation channel long transient receptor potential channel 2 (LTRPC2) by hydrogen peroxide. A splice variant reveals a mode of activation independent of ADP-ribose."Wehage E., Eisfeld J., Heiner I., Juengling E., Zitt C., Lueckhoff A.J. Biol. Chem. 277:23150-23156(2002). [2]"A novel TRPM2 isoform inhibits calcium influx and susceptibility to cell death." Zhang W., Chu X., Tong Q., Cheung J.Y., Conrad K., Masker K., Miller B.A. J. Biol. Chem. 278:16222-16229(2003).

Target Info

• Gene Symbol: TRPM2
• Gene Full Name: transient receptor potential cation channel subfamily M member 2
• Alias Symbols: EREG1;estrogen-responsive element-associated gene 1 protein;KNP3;long transient receptor potential channel 2;LTrpC-2;LTRPC2;NUDT9H;NUDT9L1;transient receptor potential cation channel subfamily M member 2;transient receptor potential channel 7;transient receptor potential melastatin 2;TRPC7.
• NCBI Gene Id: 7226
• Protein Name: Transient receptor potential cation channel subfamily M member 2
• Description of Target: Isoform 1: Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed:11960981, PubMed:12594222, PubMed:11385575, PubMed:11509734, PubMed:11804595, PubMed:15561722, PubMed:16601673, PubMed:19171771, PubMed:20660597, PubMed:27383051, PubMed:27068538). Extracellular calcium passes through the channel and increases channel activity by binding to the cytoplasmic domain and stabilizing the channel in an open conformation (PubMed:19171771). Also contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed:19454650). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (PubMed:25562606, PubMed:27068538). Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:16601673). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:11960981, PubMed:11385575, PubMed:11509734, PubMed:11804595, PubMed:15561722, PubMed:16601673, PubMed:19171771, PubMed:27383051, PubMed:27068538). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate (PubMed:20650899, PubMed:25918360). Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (PubMed:16601673). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius) (PubMed:22493272). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (By similarity). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (By similarity). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its role in increasing cytoplasmic Ca(2+) and Zn(2+) levels (PubMed:27068538). Confers susceptibility to cell death following oxidative stress (PubMed:12594222, PubMed:25562606).
• Uniprot ID: O94759
• Protein Accession #: NP_001307279
• Nucleotide Accession #: NM_001320350