TRPC6 ELISA Kit (Human) (OKCD00985)

Catalog No: OKCD00985 (Formerly GWB-KBBPG2)

Size:96WELLS

Human TRPC6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TRPC6 Microplate: 96 Wells (12 x 8 Well Strips)
  • TRPC6 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated TRPC6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6). No significant cross-reactivity or interference between Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TRPC6
• Gene Full Name: transient receptor potential cation channel subfamily C member 6
• Alias Symbols: focal segmental glomerulosclerosis 2;FSGS2;short transient receptor potential channel 6;transient receptor protein 6;TRP6;TRP-6.
• NCBI Gene Id: 7225
• Protein Name: Short transient receptor potential channel 6
• Description of Target: Thought to form a receptor-activated non-selective calcium permeant cation channel (PubMed:19936226, PubMed:23291369). Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C (PubMed:26892346). Seems not to be activated by intracellular calcium store depletion.
• Uniprot ID: Q9Y210
• Protein Accession #: NP_004612
• Nucleotide Accession #: NM_004621.5
• Protein Size (# AA): 931