TRPC3 Polyclonal Antibody (OABB00705)

Catalog No: OABB00705 (Formerly GWB-BBP628)

Size:100UG

Lane :1: Rat Liver Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Instestin Tissue Lysate
Lane 4: Rat Ovary Tissue Lysate
Lane 5:U87 Cell Lysate
Lane 6: A549 Cell Lysate
Lane7: Colo320 Cell Lysate
Lane8:SW620 Cell Lysate
Lane9: Hela Cell Lysate

IHC(P):Mouse Brain Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Hamster, Human, Mouse, Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry, Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Short transient receptor potential channel 3 (TrpC3) also known as transient receptor protein 3 (TRP-3) is a protein that in humans is encoded by the TRPC3 gene. The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene.
  Background: TRPC3(Transient receptor potential cation channel, subfamily C, member 3) also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, TRPC3 behaves as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated TRPC3 transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. TRPC3 is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TRPC3 (836-851aa HSFNSILNQPTRYQQI), identical to the related rat and mouse sequences.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
• Reference: 1. Becker, E. B. E., Oliver, P. L., Glitsch, M. D., Banks, G. T., Achilli, F., Hardy, A., Nolan, P. M., Fisher,
  E. M. C., Davies, K. E. A point mutation in TRPC3 causes abnormal Purkinje cell development and
  cerebellar ataxia in moonwalker mice. Proc. Nat. Acad. Sci. 106: 6706-6711, 2009.
  2. Philipp, S., Strauss, B., Hirnet, D., Wissenbach, U., Mery, L., Flockerzi, V., Hoth, M. TRPC3
  mediates T-cell receptor-dependent calcium entry in human T-lymphocytes. J. Biol. Chem. 278:
  26629-26638, 2003.
  3. van Rossum, D. B., Patterson, R. L., Sharma, S., Barrow, R. K., Kornberg, M., Gill, D. L., Snyder, S.
  H. Phospholipase C-gamma-1 controls surface expression of TRPC3 through an intermolecular PH
  domain. Nature 434: 99-104, 2005.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Short transient receptor potential channel 3(TRPC3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: TRPC3
• Gene Full Name: transient receptor potential cation channel subfamily C member 3
• Alias Symbols: hTrp-3;SCA41;short transient receptor potential channel 3;transient receptor potential cation channel subfamily C member 3 variant c;transient receptor protein 3;TRP3.
• NCBI Gene Id: 7222
• Protein Name: Q13507
• Description of Target: Short transient receptor potential channel 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q13507
• Molecular Weight: 96009 MW