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Catalog No: OKCD01164 (Formerly GWB-KBBMY8)
Size:96WELLS
Price: $675.00
SKU
OKCD01164
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Datasheets/ManualsPrintable datasheet for TRPA1 ELISA Kit (Human) (OKCD01164)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TRPA1 Microplate96 Wells (12 x 8 Well Strips)
TRPA1 Lyophilized Standard2 x 40 ng
100X Biotinylated TRPA1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.251 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1). No significant cross-reactivity or interference between Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTRPA1
Gene Full Nametransient receptor potential cation channel subfamily A member 1
Alias SymbolsANKTM1;ankyrin-like with transmembrane domains 1;Ankyrin-like with transmembrane domains protein 1;FEPS;FEPS1;transformation-sensitive protein p120;transient receptor potential cation channel subfamily A member 1;wasabi receptor.
NCBI Gene Id8989
Protein NameTransient receptor potential cation channel subfamily A member 1
Description of TargetReceptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function (PubMed:25389312, PubMed:25855297). Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, cinnamaldehyde, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes (PubMed:25389312, PubMed:20547126). Is also activated by menthol (in vitro)(PubMed:25389312). Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana (PubMed:25389312). May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system (By similarity).
Uniprot IDO75762
Protein Accession #NP_015628
Nucleotide Accession #NM_007332.2
Protein Size (# AA)1119
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