MMP9 ELISA Kit (Dog) (OKCA02057)

Catalog No: OKCA02057

Size:96 Wells

Dog MMP9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	99	92-105
  Serum (n=4)	1:2	88	80-96
  Serum (n=4)	1:4	87	85-90
  Serum (n=4)	1:8	92	88-107

• ELISA Kit Principle: Aviva Systems Biology MMP9 ELISA Kit (Dog) (OKCA02057) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP9 captured in well.
• ELISA Kit Range: 12.5 ng/mL-800 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	85	81-89
  EDTA plasma (n=4)	97	90-105

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-MMP9 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP9 Lyophilized Standard: 2 x 800 ng
  • 100X Biotinylated MMP9 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma
• Sensitivity: 3.12 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP9
• NCBI Gene Id: 403885
• Protein Name: Matrix metalloproteinase-9
• Description of Target: Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.
• Uniprot ID: O18733
• Protein Accession #: NP_001003219
• Nucleotide Accession #: NM_001003219