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Mouse IgG Antibody - TRITC Conjugated (OABB00877)

0.5mg
$180.00
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Host:
Goat
Application:
ICC, IF, IHC
Immunogen:
Mouse IgG (whole molecular).
Predicted Homology Based on Immunogen Sequence:
This TRITC conjugated antibody is specific for mouse IgG and shows. No cross-reactivity with mouse IgA, IgM and other animal species IgG proteins
Product Format:
Concentrated, Liquid
Reconstitution and Storage:
At 4C for one year.
Concentration:
2mg/ml
Datasheets/Manuals:
Printable datasheet for OABB00877
Specificity:
IgG
Dilution:
ICC 1:32-64 Indirect IF 1:32-64 IHC 1:32-64
Optimal working dilutions must be determined by end user
Additional Information:
Content: 0.5 mg of TRITC conjugated specific antibody (purity is above 99%), TRITC : Ab = 4-6 : 1; 0.01M PBS; 0.01% Thimerosal. The emission and filtration wavelength of TRITC are 550 nm and 620 nm respectively
:::
Diluent Buffer: Add reagent grade BSA into 0.01 M PBS (PH7.2-7.6) or TBS buffer and make BSA at a concentration of 1%. Use the above diluent buffer to dilute. See "Recommended Dilutions" below for details. Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Labeling Method: Goat anti-mouse IgG is conjugated to TRITC by means of a method described by Hijmans,W.,et al. (Reference: Hijmans,W.,et al. Clin.Exp.Immunol.,4, 457(1969))
:::
Description: This antibody was purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG.

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