TRIM33 Antibody - middle region (ARP39214_P050)

Catalog No: ARP39214_P050

WB Suggested Anti-TRIM33 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Hela cell lysateTRIM33 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells

Sample Type:
MCF7
Primary Antibody Dilution:
4 ug/ml
Secondary Antibody:
Anti-rabbit Alexa 546
Secondary Antibody Dilution:
2 ug/ml
Gene Name:
TRIM33

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IF, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRIM33
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: EIYSDRTFAPLPEFEQEEDDGEVTEDSDEDFIQPRRKRLKSDERPVHIK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-TRIM33 (ARP39214_P050) antibody is Catalog # AAP39214 (Previous Catalog # AAPY00826)
• Sample Type Confirmation:

  TRIM33 is strongly supported by BioGPS gene expression data to be expressed in HeLa

• Reference: He,W., (2006) Cell 125 (5), 929-941

Target Info

• Gene Symbol: TRIM33
• Gene Full Name: Tripartite motif containing 33
• Alias Symbols: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA
• NCBI Gene Id: 51592
• Protein Name: E3 ubiquitin-protein ligase TRIM33
• Description of Target: TRIM33 is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.
• Uniprot ID: Q9UPN9
• Protein Accession #: NP_056990
• Nucleotide Accession #: NM_015906
• Protein Size (# AA): 1127
• Molecular Weight: 122kDa
• Protein Interactions: DHX33; UBE2N; UBE2E1; UBE2D3; CHD1L; UBE2I; SUMO2; SUMO3; SKIL; TRIM24; SUMO1; MDM2; ZBTB1; SMAD4; SMAD3; SHFM1; CRYAB; SMAD2; SOX2; UBC; ANAPC1; ANAPC7; ANAPC5; ANAPC4; CDC16; CDC23; EPAS1; CDH1; CDC27; CDC20; CCNA2; BUB1B; LDB1; Nfe2l2; CUL3; ELAVL1; HI