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| Datasheets/Manuals | Printable datasheet for anti-TRIM27 (ARP34700_P050) antibody |
|---|
| Tested Species Reactivity | Human |
|---|---|
| Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB |
| Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human TRIM27 |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 86%; Pig: 100%; Rabbit: 100%; Rat: 92% |
| Peptide Sequence | Synthetic peptide located within the following region: EQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLY |
| Concentration | 0.5 mg/ml |
| Blocking Peptide | For anti-TRIM27 (ARP34700_P050) antibody is Catalog # AAP34700 (Previous Catalog # AAPP23649) |
| Gene Symbol | TRIM27 |
|---|---|
| Gene Full Name | Tripartite motif containing 27 |
| Alias Symbols | RFP, RNF76 |
| NCBI Gene Id | 5987 |
| Protein Name | Zinc finger protein RFP |
| Description of Target | TRIM27 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. |
| Uniprot ID | Q5RJA8 |
| Protein Accession # | NP_112212 |
| Nucleotide Accession # | NM_030950 |
| Protein Size (# AA) | 358 |
| Molecular Weight | 41kDa |
| Protein Interactions | LGALS14; FAM214A; USE1; CCDC94; ZNF446; TBC1D22B; ABCF3; RBM41; CCDC87; C14orf105; RBM23; WDYHV1; CHCHD3; KCTD9; FAM193B; CCHCR1; FAM64A; FBXW5; ZNF581; AMOTL2; WT1-AS; SLC15A3; CDKL3; VPS28; MEMO1; TPRKB; ARHGEF3; NME7; BABAM1; MAT2B; INPP5J; FOXB1; CDK1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "TRIM27 Antibody - middle region (ARP34700_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit".
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How long will it take to receive "TRIM27 Antibody - middle region (ARP34700_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "TRIM27 Antibody - middle region (ARP34700_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "TRIM27 Antibody - middle region (ARP34700_P050)"?
This target may also be called "RFP, RNF76" in publications.
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What is the shipping cost for "TRIM27 Antibody - middle region (ARP34700_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "TRIM27 Antibody - middle region (ARP34700_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "TRIM27 Antibody - middle region (ARP34700_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "41kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "TRIM27 Antibody - middle region (ARP34700_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TRIM27"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TRIM27"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TRIM27"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TRIM27"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TRIM27"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TRIM27"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
