Catalog No: OKWB00154
Size:96T
Price: $450.00
SKU
OKWB00154
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Datasheets/ManualsPrintable datasheet for TRIM26 ELISA Kit (Human) (OKWB00154)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-105%90-93%85-98%86-98%
EDTA Plasma (n=5)89-101%84-94%83-101%86-101%
Heparin Plasma (n=5)81-97%85-96%82-96%84-99%
ELISA Kit PrincipleAviva Systems Biology TRIM26 ELISA Kit (Human) (OKWB00154) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRIM26 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TRIM26 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRIM26 captured in the well.
ELISA Kit Range0.313-20ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)86-10394
EDTA Plasma (n=5)85-10393
Heparin Plasma (n=5)86-9790
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
TRIM26 Microplate96 Wells (12 x 8 Well strips)
TRIM26 Lyophilized Standard2 x 20 ng
100X TRIM26 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.188 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolAFP
Gene Full Namealpha fetoprotein
Alias SymbolsAFPD;alpha-1-fetoprotein;alpha-fetoglobulin;alpha-fetoprotein;FETA;HPAFP.
NCBI Gene Id174
Protein NameAlpha-fetoprotein
Description of TargetBinds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Uniprot IDP02771
Protein Accession #NP_001229712
Nucleotide Accession #NM_001242783
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