TRIM23 Antibody (OAGA00006)

Catalog No: OAGA00006

Size:100 ul

Sample(30 ug of whole cell lysate)
A:HeLa S3
10% SDS PAGE
OAGA00006 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded gastric CA, using TRIM23(OAGA00006) antibody at 1:100 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23(OAGA00006) antibody (Green) at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
  Positive Controls: HeLaS3
  Cellular Localization: Endomembrane system, Golgi apparatus membrane, Lysosome membrane
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Synthetic peptide corresponding to a region within amino acids 1 and 44 of TRIM23 (Uniprot ID#P36406)
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows:
  WB: 1:500-1:3000,
  IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)],
  ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: TRIM23
• Gene Full Name: E3 ubiquitin-protein ligase TRIM23
• Alias Symbols: ARD1, ARFD1, RNF46
• NCBI Gene Id: 373
• Protein Name: E3 ubiquitin-protein ligase TRIM23
• Description of Target: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq]
• Uniprot ID: P36406
• Protein Accession #: NP_001647.1
• Molecular Weight: 64 kDa