Catalog No: OKEH00575
Price: $600.00
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TRIM22 ELISA Kit (Human) : 96 Wells (OKEH00575)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TRIM22 ELISA Kit (Human) (OKEH00575) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human TRIM22 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human TRIM22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human TRIM22 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 83%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 7.0% (n = 20)
ELISA Kit Component
TRIM22 Microplate96 Wells (12 x 8 Well strips)
TRIM22 Lyophilized Standard2
100X Biotinylated TRIM22 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.11 ng/mL
SpecificityNatural and recombinant Human E3 ubiquitin-protein ligase TRIM22
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTRIM22
Gene Full Nametripartite motif containing 22
Alias Symbols50 kDa-stimulated trans-acting factor;E3 ubiquitin-protein ligase TRIM22;GPSTAF50;RING finger protein 94;RING-type E3 ubiquitin transferase TRIM22;RNF94;staf-50;STAF50;stimulated trans-acting factor (50 kDa);tripartite binding motif 22;tripartite motif protein TRIM22;tripartite motif-containing protein 22.
NCBI Gene Id10346
Protein NameQ8IYM9
Description of TargetE3 ubiquitin-protein ligase TRIM22
Uniprot ID
Protein Accession #NP_001186502.1
Nucleotide Accession #NM_001199573.1
Protein Size (# AA)498
Molecular Weight57 kDa
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