TRIB3 Antibody - N-terminal region (OAAB17543)

Catalog No: OAAB17543

Size:400ul

The anti-NPK Pab (Cat. # OAAB17543) is used in Western blot to detect NPK in Jurkat cell lysate.

Western blot analysis of NPK(arrow) using rabbit polyclonal NPK Antibody (Cat.# OAAB17543).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRIB3 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: TRIB3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress
  Cellular Location: Nucleus (Probable).
  Tissue Location: Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TRIB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human TRIB3.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: TRIB3
• Alias Symbols: NIPK, SINK, TRB3, SKIP3, C20orf97
• NCBI Gene Id: 57761
• Protein Name: Tribbles homolog 3
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q96RU7
• Protein Accession #: NP_066981.2
• Molecular Weight: 39578 Da