Catalog No: OKCD06887
Size:96WELLS
Price: $900.00
SKU
OKCD06887
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TRH ELISA Kit (Rat) (OKCD06887)

Datasheets/ManualsPrintable datasheet for TRH ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyrotropin Releasing Hormone (TRH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone (TRH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyrotropin Releasing Hormone (TRH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thyrotropin Releasing Hormone (TRH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyrotropin Releasing Hormone (TRH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyrotropin Releasing Hormone (TRH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
TRH Microplate96 Wells (12 x 8 Well strips)
TRH Lyophilized Standard2
100X TRH HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Gene SymbolTrh
Gene Full Namethyrotropin releasing hormone
Alias Symbolsprothyroliberin;pro-thyrotropin-releasing hormone;protirelin;Pro-TRH;THR;thyrotropin releasing hormone;thyrotropin-releasing factor;Trf;TRH01;TSH-releasing factor.
NCBI Gene Id25569
Protein NameP01150
Description of TargetPro-thyrotropin-releasing hormone
Uniprot IDhttps://www.uniprot.org/uniprot/P01150
Protein Accession #NP_037178.1
Nucleotide Accession #NM_013046.3
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