TREM1 ELISA Kit (Pig) (OKCD02955)

Catalog No: OKCD02955

Size:96WELLS

Pig TREM1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-99%	91-99%	94-103%	97-105%
  EDTA Plasma (n=5)	89-103%	81-98%	78-93%	86-95%
  Heparin Plasma (n=5)	98-105%	98-105%	90-102%	82-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Triggering Receptor Expressed On Myeloid Cells 1 (TREM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-93	85
  EDTA Plasma (n=5)	92-101	97
  Heparin Plasma(n=5)	86-97	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TREM1 Microplate: 96 Wells (12 x 8 Well strips)
  • TREM1 Lyophilized Standard: 2
  • 100X Biotinylated TREM1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 12.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Triggering Receptor Expressed On Myeloid Cells 1 (TREM1). No significant cross-reactivity or interference between Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TREM1
• Gene Full Name: triggering receptor expressed on myeloid cells 1
• Alias Symbols: TREM-1;triggering receptor expressed on myeloid cells 1.
• NCBI Gene Id: 396601
• Protein Name: Triggering receptor expressed on myeloid cells 1
• Description of Target: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock (By similarity).
• Uniprot ID: Q6TYI6
• Protein Accession #: NP_998921.1
• Nucleotide Accession #: NM_213756.1
• Protein Size (# AA): 238