Catalog No: OPED00146
Size:200 ug
Price: $593.00
SKU
OPED00146
Availability: Domestic: within 1-2 week delivery | International: 1-2 week
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Datasheets/Manuals | Printable datasheet for TRAP1 Recombinant Protein (Human) (OPED00146) (OPED00146) |
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Predicted Species Reactivity | Homo sapiens|Human |
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Product Format | Liquid. In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 1mM DTT, and 1mM EDTA. |
Host | E. Coli |
Application | WB |
Additional Information | The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress. It includes cytoplasmic (Hsp90-alpha /beta ), ER (Grp94) and mitochondrial (TRAP1) localized members. Hsp90 is structurally composed of an N-terminal ATP binding domain, a medial substrate-binding region, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones to stabilize a multitude of client proteins substrates. TRAP1 (aka Hsp75) was originally identified as a chaperone binding partner for retinoblastoma protein. TRAP1 was later found by immunofluorescence to be localized to the mitochondria. TRAP1 is significantly more active as an ATPase due in part to the lack of regulatory elements in the C-terminus, but retains the ability to dimerize. However, it does not form stable complexes with typical cochaperones of Hsp90 like p23 and HOP4. TRAP1 has been identified as a protective element for cell survival, and has been suggested as a target for anti-cancer therapeutics. |
Reconstitution and Storage | Store at -80C |
Purification | Purified by multi-step chromatography. |
Purity | >95% (SDS-PAGE; Western blot) |
Application Info | Western blot control. |
Gene Symbol | TRAP1 |
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Gene Full Name | TNF receptor associated protein 1 |
Alias Symbols | heat shock protein 75 kDa, mitochondrial;HSP 75;HSP75;HSP90L;testicular tissue protein Li 209;TNFR-associated protein 1;TRAP-1;tumor necrosis factor type 1 receptor-associated protein. |
NCBI Gene Id | 10131 |
Protein Name | Heat shock protein 75 kDa, mitochondrial |
Description of Target | Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, downstream of PINK1 and mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. |
Uniprot ID | Q12931 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 72 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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