Trap1 Antibody : Biotin (OASE00139-Biotin)

Catalog No: OASE00139-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human.
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: Trap1-6
• Isotype: IgG2a Kappa
• Host: Mouse
• Conjugation: Biotin
• Application: WB
• Additional Information: Background Info: Detects Trap1. Detects ~75kDa.
  Scientific Background: The 90 kDa heat shock protein (hsp90) family of proteins that play an important physiological role. Hsp90
  is involved in numerous cellular processes but is best
  known for its association with signal transduction
  machinery. A recently cloned homolog of hsp90 is the
  tumor necrosis factor receptor-associated protein
  (TRAP1). Like hsp90, TRAP1 is found to be associated with
  numerous proteins involved in diverse actions (1, 2).
  Immunofluorescence data has shown TRAP1 to be
  localized in the mitochondria of mammalian cells.
  This observation and the fact that TRAP1 is shown to have
  a mitochondrial targeting pre-sequence strongly implicates
  TRAP1 as a mitochondrial matrix protein (3).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of Trap-1/Hsp75 in 20 ug of Hela Cell lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Purified recombinant TRAP1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Felts S.J., et al. (2000) J Biol Chem. 275(5): 3305-3312.
  2. Costantino E., et al. (2009) Cancer Lett. 279(1): 39-
  46.
  3. Cechetto J.D., Gupta R.S. (2000) Exp Cell Res.
  260(1): 30-39.

Target Info

• Gene Symbol: TRAP1
• Gene Full Name: TNF receptor-associated protein 1
• Alias Symbols: HSP75, HSP 75, HSP90L, TRAP-1
• NCBI Gene Id: 10131
• Uniprot ID: Q12931
• Protein Accession #: NP_057376.2