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Catalog No: OKIA00085 (Formerly GWB-36931E)
Size:1x96WELLPLATE
Price: $536.00
SKU
OKIA00085
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration70 Minutes
ELISA Kit PrincipleAviva Systems Biology Transferrin ELISA Kit (Human) (OKIA00085) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serotransferrin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serotransferrin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serotransferrin captured in the well.
ELISA Kit Range9.375 ng/ml - 600 ng/ml
ELISA Kit Component
ComponentAmount
Anti-Human Transferrin Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Human Transferrin Calibrator1
Additional InformationIntended Use: The Transferrin test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Transferrin in human biological samples.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Human Transferrin antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-HUMAN TRANSFERRIN ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human TX.
7. HUMAN TRANSFERRIN CALIBRATOR
One vial containing a lyophilized Human Transferrin calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity2.551 ng/ml
Predicted Homology Based on Immunogen SequenceHuman
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTF
Gene Full Nametransferrin
Alias SymbolsTFQTL1, PRO1557, PRO2086, HEL-S-71p
NCBI Gene Id7018
Protein NameSerotransferrin
Description of TargetTransferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Uniprot IDP02787
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