TRADD Antibody - middle region (OAAB08205)

Catalog No: OAAB08205

Size:400ul

TRADD Antibody (Center) (Cat. #OAAB08205) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TRADD antibody detected the TRADD protein (arrow).

TRADD Antibody (Center) (Cat. #OAAB08205)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRADD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

TRADD Antibody (Center) (Cat. #OAAB08205) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of TRADD Antibody (Center)(Cat#OAAB08205) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: FC, IHC, IHC-P
• Additional Information: Precautions: TRADD Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B
  Cellular Location: Nucleus (By similarity). Cytoplasm (By similarity). Cytoplasm, cytoskeleton. Note=Shuttles between the cytoplasm and the nucleus (By similarity)
  Tissue Location: Found in all examined tissues.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This TRADD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-151 amino acids from the Central region of human TRADD.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: TRADD
• Alias Symbols: Hs.89862
• NCBI Gene Id: 8717
• Protein Name: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q15628
• Protein Accession #: NP_003780.1
• Molecular Weight: 34247 Da