TPX2 Antibody - C-terminal region (ARP46176_P050)

Catalog No: ARP46176_P050

WB Suggested Anti-TPX2 Antibody Titration: 0.2-1 ug/ml
Positive Control: 721_B cell lysateTPX2 is supported by BioGPS gene expression data to be expressed in 721_B

Application: Western blotting
Species + Tissue/Cell type: HeLa cell extract 20000 cells/lane, Xenopus laevis egg extract 0.3 ul/lane
How many ug's of tissue/cell lysate run on the gel:
1. HeLa cell extract 20000 cells/lane
2. Xenopus laevis egg extract 0.3 ul/lane
Primary antibody dilution: 1:1000
Secondary antibody: Anti-Rabbit HRP
Secondary antibody dilution: 1:5000

Product Info

• Tested Species Reactivity: Human, Frog
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TPX2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: LSGSLVQEPFQLATEKRAKERQELEKRMAEVEAQKAQQLEEARLQEEEQK
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-TPX2 (ARP46176_P050) antibody is Catalog # AAP46176 (Previous Catalog # AAPP27013)
• Sample Type Confirmation:

  TPX2 is supported by BioGPS gene expression data to be expressed in 721_B

• Reference: Szczepanowski,M., (2007) Biochem. Biophys. Res. Commun. 362 (2), 485-490

Target Info

• Gene Symbol: TPX2
• Gene Full Name: TPX2, microtubule-associated, homolog (Xenopus laevis)
• Alias Symbols: DIL2, p100, DIL-2, HCTP4, FLS353, HCA519, REPP86, C20orf1, C20orf2, GD:C20orf1
• NCBI Gene Id: 22974
• Protein Name: Targeting protein for Xklp2
• Description of Target: TPX2 binding decreases the size and accessibility of a hydrophobic pocket, adjacent to the ATP site, to inhibitors.
• Uniprot ID: Q9ULW0
• Protein Accession #: NP_036244
• Nucleotide Accession #: NM_012112
• Protein Size (# AA): 747
• Molecular Weight: 86kDa
• Protein Interactions: GOLGA2; UBC; RPA3; RPA2; RPA1; LMNA; HDAC9; AURKB; IKBKG; AURKA; IKBKB; CHUK; THAP11; NAP1L1; CDK6; MRPL53; ZNF598; SARNP; THUMPD2; UBE2S; DNAJC8; PPIF; SCAMP3; TWF1; NDUFA7; SERPINH1; SIAH2; HMMR; BRCA1; SUMO2; Mad2l1; KPNB1; KPNA1; FZR1; TPX2;