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Catalog No: OKCD00354 (Formerly GWB-KBBIG4)
Size:96WELLS
Price: $675.00
SKU
OKCD00354
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Datasheets/ManualsPrintable datasheet for TPP1 ELISA Kit (Human) (OKCD00354)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tripeptidyl Peptidase I (TPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tripeptidyl Peptidase I (TPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tripeptidyl Peptidase I (TPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tripeptidyl Peptidase I (TPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tripeptidyl Peptidase I (TPP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tripeptidyl Peptidase I (TPP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TPP1 Microplate96 Wells (12 x 8 Well Strips)
TPP1 Lyophilized Standard2 x 10 ng
100X Biotinylated TPP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tripeptidyl Peptidase I (TPP1). No significant cross-reactivity or interference between Tripeptidyl Peptidase I (TPP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTPP1
Gene Full Nametripeptidyl peptidase 1
Alias Symbolscell growth-inhibiting gene 1 protein;CLN2;GIG1;growth-inhibiting protein 1;LPIC;lysosomal pepstatin insensitive protease;lysosomal pepstatin-insensitive carboxypeptidase;Lysosomal pepstatin-insensitive protease;SCAR7;TPP-1;tripeptidyl aminopeptidase;tripeptidyl peptidase I;tripeptidyl-peptidase 1;Tripeptidyl-peptidase I.
NCBI Gene Id1200
Protein NameTripeptidyl-peptidase 1
Description of TargetLysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus (By similarity).
Uniprot IDO14773
Protein Accession #NP_000382
Nucleotide Accession #NM_000391.3
Protein Size (# AA)563
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