TPM3 ELISA Kit (Human) (OKCD00907)

Catalog No: OKCD00907 (Formerly GWB-KBBLQ6)

Size:96WELLS

Human TPM3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-96%	94-101%	95-104%	96-105%
  EDTA plasma(n=5)	79-97%	89-104%	96-103%	93-101%
  heparin plasma(n=5)	87-104%	90-104%	96-105%	90-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tropomyosin 3 (TPM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tropomyosin 3 (TPM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tropomyosin 3 (TPM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tropomyosin 3 (TPM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	102
  EDTA plasma(n=5)	91-105	101
  heparin plasma(n=5)	93-102	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tropomyosin 3 (TPM3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tropomyosin 3 (TPM3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-TPM3 Microplate: 96 Wells (12 x 8 Well Strips)
  • TPM3 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated TPM3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tropomyosin 3 (TPM3). No significant cross-reactivity or interference between Tropomyosin 3 (TPM3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: TPM3
• Gene Full Name: tropomyosin 3
• Alias Symbols: alpha-tropomyosin, slow skeletal;CAPM1;CFTD;cytoskeletal tropomyosin TM30;epididymis luminal protein 189;epididymis secretory sperm binding protein Li 82p;Gamma-tropomyosin;heat-stable cytoskeletal protein 30 kDa;HEL-189;HEL-S-82p;hscp30;NEM1;OK/SW-cl.5;TM3;TM30;TM30nm;TM5;TM-5;TPM3nu;TPMsk3;TRK;tropomyosin 3 nu;tropomyosin alpha-3 chain;tropomyosin gamma;Tropomyosin-3;tropomyosin-5.
• NCBI Gene Id: 7170
• Protein Name: Tropomyosin alpha-3 chain
• Description of Target: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
• Uniprot ID: P06753
• Protein Accession #: NP_001036816
• Nucleotide Accession #: NM_001043351.1
• Protein Size (# AA): 285