Catalog No: OKCD01702 (Formerly GWB-KBBLU2)
Size:96WELLS
Price: $675.00
SKU
OKCD01702
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for TP63 ELISA Kit (Human) (OKCD01702) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein P63 (TP63). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein P63 (TP63). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein P63 (TP63), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Protein P63 (TP63) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein P63 (TP63) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein P63 (TP63) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.057 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Tumor Protein P63 (TP63). No significant cross-reactivity or interference between Tumor Protein P63 (TP63) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | TP63 |
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Gene Full Name | tumor protein p63 |
Alias Symbols | AIS;amplified in squamous cell carcinoma;B(p51A);B(p51B);chronic ulcerative stomatitis protein;EEC3;keratinocyte transcription factor KET;KET;LMS;NBP;OFC8;p40;p51;p53CP;p63;p73H;p73L;RHS;SHFM4;TP53CP;TP53L;TP73L;transformation-related protein 63;tumor protein 63;tumor protein p53-competing protein;Tumor protein p73-like. |
NCBI Gene Id | 8626 |
Protein Name | Tumor protein 63 |
Description of Target | Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. |
Uniprot ID | Q9H3D4 |
Protein Accession # | NP_001108450.1 |
Nucleotide Accession # | NM_001114978.1 |
Protein Size (# AA) | 680 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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