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Catalog No: OKCD01702 (Formerly GWB-KBBLU2)
Size:96WELLS
Price: $675.00
SKU
OKCD01702
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Datasheets/ManualsPrintable datasheet for TP63 ELISA Kit (Human) (OKCD01702)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein P63 (TP63). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein P63 (TP63). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein P63 (TP63), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tumor Protein P63 (TP63) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Protein P63 (TP63) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Protein P63 (TP63) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-TP63 Microplate96 Wells (12 x 8 Well Strips)
TP63 Lyophilized Standard2 x 10 ng
100X Biotinylated TP63 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Tumor Protein P63 (TP63). No significant cross-reactivity or interference between Tumor Protein P63 (TP63) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolTP63
Gene Full Nametumor protein p63
Alias SymbolsAIS;amplified in squamous cell carcinoma;B(p51A);B(p51B);chronic ulcerative stomatitis protein;EEC3;keratinocyte transcription factor KET;KET;LMS;NBP;OFC8;p40;p51;p53CP;p63;p73H;p73L;RHS;SHFM4;TP53CP;TP53L;TP73L;transformation-related protein 63;tumor protein 63;tumor protein p53-competing protein;Tumor protein p73-like.
NCBI Gene Id8626
Protein NameTumor protein 63
Description of TargetActs as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.
Uniprot IDQ9H3D4
Protein Accession #NP_001108450.1
Nucleotide Accession #NM_001114978.1
Protein Size (# AA)680
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