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Catalog No: OKEH08564
Size:96T
Price: $725.00
SKU
OKEH08564
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology TP53I3 ELISA Kit (Human) (OKEH08564) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TP53I3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TP53I3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TP53I3 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.7% (n = 20)
Mean Inter-assay CV%: <=8.1% (n = 20)
ELISA Kit Component
ComponentAmount
TP53I3 Microplate96 Wells (12 x 8 Well strips)
TP53I3 Lyophilized Standard2
100X Biotinylated TP53I3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent beta-naphthoquinone reductase activity, with a preference for 1,2-beta-naphthoquinone over 1,4-beta-naphthoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).
::Unigene: Hs.50649
SMR: Q53FA7
String: 9606.ENSP00000238721
::KEGG: hsa:9540
MIM: 605171
Pfam: PF08240
Reconstitution and StorageStore as indicated in product manual.
Sensitivity30pg/mL
SpecificityNatural and recombinant Human Quinone oxidoreductase PIG3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTP53I3
Gene Full Nametumor protein p53 inducible protein 3
Alias Symbolsp53-induced gene 3 protein;PIG3;quinone oxidoreductase homolog;quinone oxidoreductase PIG3;Tumor protein p53-inducible protein 3.
NCBI Gene Id9540
Protein NameQ53FA7
Description of TargetQuinone oxidoreductase PIG3
Uniprot IDhttps://www.uniprot.org/uniprot/Q53FA7
Protein Accession #NP_001193731.1
Nucleotide Accession #NM_001206802.2
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