TP53 ELISA Kit (Bovine) (OKEH07503)

Catalog No: OKEH07503

Size:96T

Bovine TP53 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology TP53 ELISA Kit (Bovine) (OKEH07503) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TP53 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TP53 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TP53 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.0% (n = 20)
  Mean Inter-assay CV%: <=11.5% (n = 20)
• ELISA Kit Component:
  • TP53 Microplate: 96 Wells (12 x 8 Well strips)
  • TP53 Lyophilized Standard: 2
  • 100X Biotinylated TP53 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
  Subcellular Location: Cytoplasm Nucleus Endoplasmic reticulum Mitochondrion matrix Interaction with BANP promotes nuclear localization. Translocates to mitochondria upon oxidative stress (By similarity).
  Unigene: Bt.49712
  SMR: P67939
  String: 9913.ENSBTAP00000001420
  KEGG: bta:281542
  Pfam: PF00870
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 12.7pg/mL
• Specificity: Natural and recombinant Bovine Cellular tumor antigen p53
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: TP53
• Gene Full Name: tumor protein p53
• Alias Symbols: cellular tumor antigen p53;p53 tumor suppressor phosphoprotein;tumor protein 53;tumor protein p53 (Li-Fraumeni syndrome);tumor suppressor p53.
• NCBI Gene Id: 281542
• Protein Name: P67939
• Description of Target: Cellular tumor antigen p53
• Uniprot ID: https://www.uniprot.org/uniprot/P67939
• Protein Accession #: NP_776626.1
• Nucleotide Accession #: NM_174201.2