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Datasheets/Manuals | Printable datasheet for TP53 Antibody (OAEE01088) (OAEE01088) |
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Predicted Species Reactivity | Human|Non-human Primate |
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Product Format | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Clonality | Monoclonal |
Clone | BP53-12 |
Isotype | IgG2a |
Host | Mouse |
Conjugation | FITC |
Application | Flow cytometry |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | Bacterially expressed full-length wild-type p53 |
Purification | Purified by size-exclusion chromatography |
Concentration | 1 mg/ml |
Specificity | The antibody BP53-12 recognizes defined epitope (aa 16-25) on human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells; it is frequently mutated or inactivated in many types of cancer. |
Formulation | FITC |
Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
Reference | Dolezalova H, Vojtesek B, Kovarik J: Epitope analysis of the human p53 tumour suppressor protein. Folia Biol (Praha). 1997;43(1):49-51. Bartkova J, Bartek J, Lukas J, Vojtesek B, Staskova Z, Rejthar A, Kovarik J, Midgley CA, Lane DP: p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia. Int J Cancer. 1991 Sep 9;49(2):196-202. Bartek J, Bartkova J, Vojtesek B, Staskova Z, Lukas J, Rejthar A, Kovarik J, Midgley CA, Gannon JV, Lane DP: Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies. Oncogene. 1991 Sep;6(9):1699-703. Tanigawa S, Fujii M, Hou DX: Stabilization of p53 is involved in quercetin-induced cell cycle arrest andapoptosis in HepG2 cells. Biosci Biotechnol Biochem. 2008 Mar;72(3):797-804. Taylor WR, Agarwal ML, Agarwal A, Stacey DW, Stark GR: p53 inhibits entry into mitosis when DNA synthesis is blocked. Oncogene. 1999 Jan 14;18(2):283-95. Agarwal ML, Agarwal A, Taylor WR, Stark GR: p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts. Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8493-7. Taylor WR, DePrimo SE, Agarwal A, Agarwal ML, Schönthal AH, Katula KS, Stark GR: Mechanisms of G2 arrest in response to overexpression of p53. Mol Biol Cell. 1999 Nov;10(11):3607-22. Agarwal ML, Agarwal A, Taylor WR, Chernova O, Sharma Y, Stark GR: A p53-dependent S-phase checkpoint helps to protect cells from DNA damage inresponse to starvation for pyrimidine nucleotides. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14775-80. |
Gene Symbol | TP53 |
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Gene Full Name | tumor protein p53 |
Alias Symbols | antigen NY-CO-13;BCC7;BMFS5;cellular tumor antigen p53;LFS1;mutant tumor protein 53;P53;p53 tumor suppressor;phosphoprotein p53;transformation-related protein 53;TRP53;tumor protein 53;Tumor suppressor p53;tumor supressor p53. |
NCBI Gene Id | 7157 |
Protein Name | Cellular tumor antigen p53 |
Description of Target | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). |
Uniprot ID | P04637 |
Protein Accession # | NP_000537.3; NP_001119584.1; NP_001119585.1; NP_001119586.1; NP_001119587.1; NP_001119588.1 |
Nucleotide Accession # | NM_000546.5; NM_001126112.2; NM_001126113.2; NM_001126114.2; NM_001126115.1; NM_001126116.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "TP53 Antibody (OAEE01088)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Non-human Primate".
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How long will it take to receive "TP53 Antibody (OAEE01088)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "TP53 Antibody (OAEE01088)" provided in?
This item is provided in "Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "TP53 Antibody (OAEE01088)"?
This target may also be called "antigen NY-CO-13;BCC7;BMFS5;cellular tumor antigen p53;LFS1;mutant tumor protein 53;P53;p53 tumor suppressor;phosphoprotein p53;transformation-related protein 53;TRP53;tumor protein 53;Tumor suppressor p53;tumor supressor p53." in publications.
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What is the shipping cost for "TP53 Antibody (OAEE01088)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "TP53 Antibody (OAEE01088)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "TP53 Antibody (OAEE01088)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "TP53 Antibody (OAEE01088)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TP53"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TP53"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TP53"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TP53"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TP53"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TP53"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.