- Predicted Species Reactivity:
- Product Format:
- 1 x 96-Well Plate or 12 x 8-Well Strips
- Kit Detection Method:
- Colorimetric, OD450 nm
- Kit Principle:
- The Aviva Human Total MMP-9 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hMMP-9 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human MMP-9 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
- Kit Range:
- 32-2,000 pg/mL
- Kit Component:
|Capture Antibody Coated Microplate||96 Wells (12 x 8)|
|Ready-to-Use Streptavidin-HRP||12 mL|
|Ready-to-Use Substrate||12 mL|
|Stop Solution||12 mL|
|Wash Buffer (15x)||50 mL|
|Protein Standard Diluent||12 mL|
|Sample Diluent||12 mL|
|Detection Antibody Diluent||12 mL|
|Biotin-Labeled Detection Antibody||Lyophilized|
|Protein Standard||Lyophilized (1.7 ng)|
- Reconstitution and Storage:
- Store as indicated in product manual.
- Sample Type:
- Cell lysates, sera and plasma
- 32 pg/mL
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- The Human MMP-9 ELISA is capable of recognizing both recombinant and naturally produced Human MMP-9 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-10, MMP-12, MMP-13, MMP-14, TIMP-2, TIMP-3, TIMP-4
• Murine: MMP-9
- Assay Info:
- Quantitative Colorimentric Sandwich ELISA
- Official Gene Full Name:
- matrix metallopeptidase 9
- Alias Symbols:
- 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, MANDP2, Matrix metalloproteinase-9, MMP-9
- NCBI Gene Id:
- Protein Name:
- Matrix metalloproteinase-9
- Description of Target:
- Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express MMP9.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express MMP9.