TOP2B Antibody - middle region (ARP38011_P050)

Catalog No: ARP38011_P050

WB Suggested Anti-TOP2B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysateTOP2B is supported by BioGPS gene expression data to be expressed in Jurkat

TOP2B was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with ARP38011_P050 with 1:200 dilution. Western blot was performed using ARP38011_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: TOP2B IP with ARP38011_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IP
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TOP2B
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: ENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGR
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-TOP2B (ARP38011_P050) antibody is Catalog # AAP38011 (Previous Catalog # AAPP20184)
• Sample Type Confirmation:

  TOP2B is supported by BioGPS gene expression data to be expressed in Jurkat

• Reference: McNamara,S., (2008) Mol. Cell. Biol. 28 (6), 2066-2077

Target Info

• Gene Symbol: TOP2B
• Gene Full Name: Topoisomerase (DNA) II beta 180kDa
• Alias Symbols: TOPIIB, top2beta
• NCBI Gene Id: 7155
• Protein Name: DNA topoisomerase 2-beta
• Description of Target: TOP2B is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: Q02880
• Protein Accession #: NP_001059
• Nucleotide Accession #: NM_001068
• Protein Size (# AA): 1621
• Molecular Weight: 183kDa
• Protein Interactions: BAZ1B; SUMO2; SUMO3; UBC; EIF6; RPA3; RPA2; RPA1; ERG; WHSC1; NOTCH1; ESR1; NCSTN; SNRNP200; RBM14; HNRNPR; HDAC3; XPC; TOP2A; SNRPE; SMARCE1; SMARCD2; SMARCC2; SMARCC1; SMARCB1; SMARCA4; SMARCA2; SFPQ; S100A9; MSH2; IK; SEPT7; SUMO1; CUL5; NEDD8; RANBP2;