Catalog No: OKEH07192
Size:96T
Price: $600.00
SKU
OKEH07192
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Top2a ELISA Kit (Mouse) (OKEH07192) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Top2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Top2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Top2a captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 110%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <6.4%
Inter-Assay CV <9.1%
ELISA Kit Component
ComponentAmount
Top2a Microplate96 Wells (12 x 8 Well strips)
Top2a Lyophilized Standard2 x 5 ng
100X Top2a HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity39.1 pg/mL
SpecificityNatural and recombinant Mouse DNA topoisomerase 2-alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTop2a
Gene Full Nametopoisomerase (DNA) II alpha
Alias SymbolsDNA topoisomerase 2-alpha;DNA Topoisomerase II alpha;DNA topoisomerase II, alpha isozyme;Top;Top-2.
NCBI Gene Id21973
Protein NameQ01320
Description of TargetDNA topoisomerase 2-alpha
Uniprot IDhttps://www.uniprot.org/uniprot/Q01320
Protein Accession #NP_035753
Nucleotide Accession #NM_011623
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