Catalog No: OKEH01846
Size:96T
Price: $600.00
SKU
OKEH01846
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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TOP2A ELISA Kit (Human) : 96 Wells (OKEH01846)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TOP2A ELISA Kit (Human) (OKEH01846) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TOP2A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TOP2A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TOP2A captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 80%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
TOP2A Microplate96 Wells (12 x 8 Well strips)
TOP2A Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated NF1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.2 ng/mL
SpecificityNatural and recombinant Human DNA topoisomerase 2-alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTOP2A
Gene Full NameDNA topoisomerase II alpha
Alias SymbolsDNA gyrase;DNA topoisomerase (ATP-hydrolyzing);DNA topoisomerase 2-alpha;DNA topoisomerase II, 170 kD;DNA topoisomerase II, alpha isozyme;TOP2;TOP2alpha;TOPIIA;topoisomerase (DNA) II alpha 170kDa;TP2A.
NCBI Gene Id7153
Protein NameP11388
Description of TargetDNA topoisomerase 2-alpha
Uniprot IDhttps://www.uniprot.org/uniprot/P11388
Protein Accession #NP_001058.2
Nucleotide Accession #NM_001067.3
Protein Size (# AA)1531
Molecular Weight174 kDa
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