Catalog No: OOPA00090 (Formerly GWB-BSP320)
Size:5UG
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OOPA00090 |
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Host | Sf9 insect cells. |
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:: | Product Introduction: TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name. Product Description: Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag. |
Purity | Greater than 80% as determined by SDS-PAGE. |
Application Info | Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody. |
Formulation | TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol. |
Purification | Greater than 80% as determined by SDS-PAGE. |
Gene Symbol | TOP1 70kDa |
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Alias Symbols | DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70. |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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